1990年 · 第06卷第04期

In the present study, the percentages of leucocytes in urine and renal epithelial cells were counted under light microscope in the specimens stained with toluidineblue-parafuchsine developed by ourself. The observations were made in a total of 129 cases, which involving seven kinds of renal and urinary tract diseases.

The results obtained and the conclusions that could be reached are as follows; (1) various kinds of leucocytes in urine ahd renal epithelial cells could be distinguished by the present method. (2) A finding of granulocytes (GC) more than 85% and renal epithelial cells less than 15% could be considered as an indication for the diagnosis of acute pyelonephritis and cystitis. (3) The observation that GC are less than 30% strongly suggest the presence of glomerular diseases. (4) A finding that the sum of renal epithelial cells and degenerated renal epithelial cells amount to 50-100%, whereas GC amount to 0-50%, supports the diagnosis of primary nephrotic syndrome (NS), purpuric nephritis and lupus nephritis, in terms of diagnostic validity, the sensitivity of this observation was 96%, the specificity 100%, the positive predicted value 100%, the negative predicted value 88% and the morbidity 76.74%. (5) The count of degenerated renal epithelial cells could be higher than 30% in NS Ⅱ (6) No degenerated renal epithelial cells were observed in patients with acute nephritis, pyelonephritis and cystitis.

The activity of immuno-regulation T Lymphocytes subsets-Th-cell and Ts-cell have been investigated in patients with acute nephritis and chronic nephritis peripheral blood Th-cell activity was significantly increased compared with the normal controls in 15 acute glomerulonephritis; The activity of Ts-cell compared with the normal controls were significantly decreased in 50 chronic glomerulonephritis. The results suggest the mmuno-regulation disfunction of T-cell was presented in nephrotis, but there were significat differences both Th-cell and Ts-cell in patients with acute nephritis and chronic nephritis. It is no matter whether the change of this two regulatary T lymphocytes function is primary or secondary to the change of B Iymphocyt activict. It suggested that the imbalance of Th and Ts cells regulatary fnuclion played a very important role on the nephritis. Especially, the decrease of Ts-cell activity maybe related colsely of the chronicity of nephritis.