1994年 · 第10卷第01期

Chinese herb medicine-Cordyceps Sinensis (CS) was taken to treat the animal model of chronic renal insufficiency induced in Wistar rat by 5/6 nephrectomy. The glomeruli area were measured using a computer image analysis system and the renal histological changes of these rats were evaluated according to 7 parameters - mesangial hypercellularity, mesangial sclerosis, tuft adhesion, segmental glomerular sclerosis, interstitial fibrosis, tubular atrophy and vascular lesions. The results showed that CS decreased significantly the level of BUN and Scr in experimental rats and can inhibit the hypertrophy of glomeruli in those rats. The renal histological lesion of experimental rat was improved by treatment with CS.

Twenty-two male Wistar rats, weighing between 150 and 200 g, were divided into model control and BIm groups. The model of in-situ glomerulonephritis of rat was induced by intravenous injection of cationic bovin serum albumin (c-BSA). The rats of BIim group were injected. With BIm (5mg/kg/day), a thromboxane A₂ synthase inhibitor, from week 3 to week 4 after injection of c-BSA. The HGE2 and TXB2 (the stable breakdom product of TXA2) were significantly increased and 6-keto-PGF1α (the stable breakdown product of PGI2) was significantly decrcased in the rat renal cortexes of model control group than those in normal ones in week 4. The rat renal cortexes of BIm groug had significantly lower TXB2 and higher 6-keto-PGF1α than model control group. Protective effect on proteinuria and renal injuries was achieved in BIm group. The disordered PGI2-TXA2 balance in renal cortex may be an important factor in progression of glomerular injury.

The model of progressive passive Heymann nephritis (PPHN) in rats was established by multiple injections of heterologous antiserum against renal tubular epithelial antigen (Tub-Ag) of rats. The process and condition of PPHN were longer and more severe than those of passive Heymann nephritis (PHN). The manifestations of PPHN were similar to those of human membranous glomerulonephropathy (MGN). Moreover. the antibodies eluted gromeruli of the rats with PPHN or PHN were assayed and it was found that only the glomeruli of the rats with PPHN contained autoantibody against Tub-Ag, which could react with renal tubular brush border (BB) region of normal rats. These results suggest that the progression of PPHN not only originates from the stimulations of the heterologous antiserum, but also associates with the formation of autoantibody against Tub-Ag of rats. The autoantibody probably plays a significant role in preserving chronic progressive phase of the PPHN in rats.