1992年 · 第08卷第06期

T cell subpopulations in the interstitium of 78 renal biopsy specimens from patients with lupus nephritis were studied with monoclonal antibodies and 4-layer PAP method. The result is summarized as following.

1. The numbers of interstitial CD₄⁺ T helper/inducer lymphocytes and CD8⁺ cytotoxic/suppressor cells in lupus nephritis were remarkably greater than that of normal control, being more pronounced in the active lesion.

2. The RT value (CD₄+/CD₈⁺) of infiltrating T cells was less than 1 in most cases, but specimen of active lesion tended to have a higher value.

3. Patients with active lesion often showed CD₄⁺focal clusters with an increased RT value. Glomerulosclerosis, crescents formation and impairment of renal function were common in these occassions.

It was concluded that T cell subpopulation in renal interstitium might be served as an index for lupus activity and predictor of the disease.

The purpose of this experimental study is to investigate the relation between the changes of TXA2-PGI2 balance and glomerular injury, and the effects of Dazoxiben, Chuan Xiong on in situ immune complex glomerulonephritis (ISICGN) produced by C-BSA in rats.

Our results have suggested that there is a TXA2-PGI2 imbalance in ISICGN, TXA₂ play an important pathogenetic role in the onset and progression of glomerulonephritis. Dazoxben and Chuan Xiong might improve TXA2-PGI2 imbalance, and attenuate glomerular injury to some extent in ISICGN.

Nephrotic syndrome of animal model was produced by intravenously injection of adriamycin 5mg/kg to 24 Wistar male rats. The rats were paired according to weight and divided into experimental and control groups. Eight pairs were observed for 2 weeks and 4 pairs for 4 weeks. In experimental (E) group, the proteinuria reached maximum on 10th day after injection. At the same time, they showed hypoalbuminemia, hypercholesterolemia, decrease of serum sodium concentration and urinary sodium excretion. When compared with control (C) group, all of them showed significant differences. Under electron microscopy, in E groups, they all showed foot process fusion of epithelial cells, especially in the group of observation of 4 weeks. Using injection of a silicone rubber containing Chromium into renal artery, the glomerular capillary diameters were measured, they were also obviously declined in E groups both in observation of 2 and 4 weeks than that in C groups (p<0.005 and 0.01 respectively).

Then 50 of glomeruli microdissected from each animal were exposed to flux of 2×10¹³ neutrons. cm^-2·sec^-1 for 2 hours. After that the glomerular surface area (S) were calculated and they showed decrement in E group of 4 weeks than that in C group (p<0.05). Since K=K×S, these suggested Kf in E group maybe also declined.