1992年 · 第08卷第01期

An autoimmune glomerulonephritis in Brown Norway rat was established by several successive subcutaneous injections with O.lmg/100g body weight HgCl₂. Several techniques including proteinuria quantitation, immunofluorescence, electron microscopy and immunogold of electron microscopy were used to study the model. It was found that Brown Norway rats developed a biphasic autoimmune disease characterized by both anti-GBM antibodys and immune complex deposits. The pathological process of the disease underwent four stages, each stage had its pathological characteristics. This might be an ideal model for the study of human glomerulonephritis in our country.

The level of α₁-M in the sera and urines were determined by RIA in 42 patients with various renal diseases and compared with those of β₂-microglobulin (β₂-M), 24 hours creatinine clearance (CCr) and creatinine (Cr) to evaluate its clinical significance. There was a significant positive correlation between sera α₁-M, β₂-M, and Cr level (P<0.01). Sera α₁-M, β₂-M and Cr inversely correlated to CCr (P<0.01). α₁-M level started to increase over normal range when CCr>70ml/min, while β₂ -M and Cr remained within normal range. These data suggested that measurement of α₁-M in the sera and urines seemed to be useful to estimate GFR and as an sensitive index of the dysfunction of renal tubules.