Volume 96  ·   Issue 09  ·  1983

10 cases of idiopathic universal calcinosis are reported. All lesions with or without symptoms were distributed in the soft tissue all over the body. Tests for blood calcium and phosphorus were made in 9 and were all normal The discussion covers classfication, X-ray appearance and pathologic changes. Brief comments on diagnosis, differential diagnosis and prognosis are made.

Two cases of cor triatriatum with complex cardiac malformations are treated surgically with gratifying results. The diagnosis, differential diagnosis and method of surgical treatment are briefly discussed.

Anopheles (An) sinensis Wiedmann, 1828, is widely distributed in all counties and cities of Henan Province. It has been proved that this is the main vector transmitting malaria and malayan filariasis. Generally new adults first appear in the middle decade of April with 2 peaks in rice producing areas and l peak in dry farming areas. Only the adults hibernate from the middle decade of November. In the first decade of March overwintering females start to leave hibernation sites and feed in cow sheds. A gonotrophic cycle is completed within an average 2.5 days during July to September and mosquitoes double in number at an average 3.5 day interval. The gonotrophic cycles are closely linked and the potential malaria transmitting capacity great. An sinensis bites both men and animals with cattle preferred. The average value of human blood index is 0.2073. Biting is prevalent throughout the night from June to September. The larvae of the first generation begin to appear in the last decade of March gradually extending from the south to the north. The limited early breeding places extend greatly in July to September when larvae are seen in all kinds of water. But the breeding places localize again after October. In the middle or the last decade of December the larvae completely disappear. The main breeding places are water bodies with floating vegetation.

Seven pairs of mixed porcine epidermal cell and human lymphocyte cultures (MSP LhC) and mixed porcine and human lymphocyte cultures (MLphC) are carried out. For comparison MSpLhC and mixed porcine lymphocyte culture (MLpC) are cultured simultaneously. The average stimulation index of MSpLhC is 7.06±2.78; of MLphC is 23.51±21.7. The experiment reveals that the human lymphocytes do not readily respond to porcine epidermal cell stimulation by blast formation which implies that xenograft skin may have a specific form of rejection reaction. This finding is consistent with the authors' clinical observations.

10 cases of WPW syndrome are diagnosed by repeated episodes of tachycardia, electrocardiogram, vectocardiogram and electrophysiologic studies. Two are associated with other cardiac diseases (atrial septal defect in 1 and mitral stenosis in the other). Interruption of the anomalous conduction pathway between the atrium and ventricle is guided by epicardial mapping, anomalous conduction pathways are cut in 7 cases, injected with alcohol in 1 and both technics are used in 2. Good results are obtained in 8, there is persistent delta wave but symptom relief in 1 and recurrence of tachycardia in 2. One unsuccessful case was reoperated upon with good results.

From 1973 to 1981 50 cases of disseminated lung cancer were treated by direct liquid nitrogen freezing technic. All these patients were unsuitable for either radiation therapy or pulmonary resection. By using this technic half of them survive over 2 years. To further evaluate cryosurgery in these patients the authors suggest the following types of lung cancer be considered for trial cryosurgery: those with poor pulmonary or cardiac function or large tumor with cavitation, where pulmonary lobectomy or radio-therapy is contraindicated; those with unilateral or bilateral metastatic lung cancer with the primary tumor under control; when the tumor found during thoracotomy is too extensive for resection, cryosurgery can be used as a palliative measure or as adjuvant therapy; cryosurgery combined with anaerobic corynebacterium vaccine is more effective than cryosurgery alone in disseminated lung cancer with pleural effusion or multiple lesions.

The authors suggest that the critical lethal freezing temperature of monolayer cells is -40 C 3 minutes, but 3 of these freeze-thaw cycles are required for S-180 sarcoma suspension lethality.

Chromosome changes are studied in 21 cases of chronic benzene poisoning, both hypodiploid and hyperdiploid cells are significantly increased. Among the hypodiploid cells, deletion of Group C, E and G chromosomes is observed. Analysis of hyperdiploid cells reveals extra Group C and E chromosomes. The chromatid gaps and breaks are mostly found in poisoned cases, but fragments and minutes are less common. The chromosome abnormalities induced by chronic benzene intoxication may be considered further proof of benzene metabolite acting chiefly through changes in the nucleic acid metabolism.

In 1978, we detected 20 cases of polyps with cancerous changes in mass screening for colorectal cancer in Haining county. The earliest stage cases did not fit Dukes' classification or the then extant Dukes' modified classification. We suggested a new modification of Dukes' classification (the Hangzhou classification) based upon anatomicopathologic changes in 165 cases (73 colon, 92 rectal) treated in our hospital and surviving more than 5 years postoperatively from 1961 to 1975. We combined the Hangzhou classification with the internationally adopted TNM classification.

By fusing NS-1 mouse myeloma cells with spleen cells from immunized BALB/c mice with MEF 1 strain type 2 poliovirus, 7 hybridoma cell lines secreting type 2 poliovirus monoclonal antibodies are established in our laboratory. These hybrid cells possess many of their parents' properties as chromosome number and morphology producing cancer nodules by intraperitoneal injection and stable production of specific antibodies. The titer of neutralizing antibodies secreted by hybrid cell in vitro cultured is 1:80-5120 and of antibodies in BALB/c mice ascitic fluid 1:20480-327680. The monoclonal antibodies are strictly type-specific, some have different neutralizing activities to different strains of the same type poliovirus.