Adult male (C57×LACA)F1 mice were irradiated with 9.5 Gy of γ-rays and injected with spleen cells, either non-incubated or incubated with placental globulin (PG) and dexamethasone (DXM). Mice of the control group were irradiated only.
All animals in the control group died within 28 days after irradiation, while all mice treated with non-incubated spleen cells died even much earlier than the controls-within 9 days after irradiation. The 30-day-survival rate of mice injected with incubated spleen cells was 77.1%.
The spleen index in mice treated with non-incubated spleen cells (2.44±0.27) was higher than that in mice injected with incubated spleen cells (1.25±0.14).
These experiments showed that PG and DXM could reduce the severeness of GVHD in the treated mice, and, therefore, increased their survival rate.
