1985年 · 第01卷第01期

In 56 Glomerulonephritis patients, C₁q-ELISA, C₁q--BA and PEG methods were used to detect the CIC levels. The positive rates were 40.4%, 40.8% and 32.1% respectively. It proved that C₁q-ELISA method is as sensitive as C₁q-BA method, it has no disadvantages of health menace, costly equipment of C₁q-BA method. It is thus a method worthwhile to be popularized in clinical use. The new method was described in detail.

During June-July, 1983, 34 cases of chronic mercury poisoning due to long-term exposure to mercury steam were examined by the authors in two hospitals. Among these 34 patients, chronic mercury toxic nephropathy were detected in 7 cases (20.6%). All these 7 cases had undergone renal biopsy. 5 were successful, 2 failed. The major renal pathological changes of these 5 patients were mesangial proliferative glomerulonephritis associated with tubulointerstitial lesions but the glomeruli were predominant sites of involvemeut.

Our conclusions are: (1) The persons who had exposed to high concentration of mercury steam environment for a long time would suffer from chronic mercury toxic nephropathy. (2) According to our data, the incidence of chronic mercury toxic nephropathy was 20.6% inpatients with chronic mercury poisoning due to long-term exposure to mercury steam. (3) In the present study, the major renal pathological changes of chronic mercury toxic nephropathy were mesangial proliferative glomerulonephritis associated with tubulointerstitial lesions. (4) The pathogenic mechanisms of chronic mercury toxic nephropathy due to long-term exposure to mercury steam remains a mystery. we should be attached importance to the mechanism of immune mediation except the effect of direct cellular toxication. The immune complex disease in situ should be considered.

The experimental model of in situ immune complex GN could be produced by IV injections of C-BSA 10 mg daily for about 2 weeks. After 2 week injections of C-BSA (experimental group), proteinuria began to appear and IgG and C₃ deposits in glomeruli could be found by immunofluorescence in all rabbits. After 6 week injections of C-BSA, the proteinuria in experimental group (n=13) was significantly greater in amount in comparison with the control group (n=12) injected with natural BSA (234.7±38.9 mg/24h VS 29.6±7.3 mg/24h, P<0.001).

We also found definite inflammatory changes in the experimental group——increased diameter of the glomeruli, presence of increased cellularity and formaton of thrombosis.

The process of making this model is comparatively simple, its pathological change is uniform and its incidence of success is high. we therfore consider this model as a practical one in experimental GN.