Research Institute of Obstetrics and Gynecology and Division of Analytical Chemistry, Department of Pharmacy, Shanghai First Medical College, Shanghai
Chinese Medical JournalVol.94,No.031981
DOI: 10.3760/cma.j.issn.0366-6999.1981.03.118
Abstract
A rapid, reliable, simple and practical fluorometric method for determining total urine estrogens is described. Three different methods of determining fluorescence reaction were com- pared and it was found that the one with 65% H2SO4 as reaction reagent produces the most stable and intensive fluorescence. The experimental conditions such as the time of processing, temperature, solvent etc. were investigated. From our results a routine procedure was designed, including the processes of hydrolysis, extraction, solvent partition and fluorescent reaction. The procedure is greatly simplified and 20-30 samples can be done simultaneously. The efficiency of estrogen recovery by this method is 87.9-113.73%, coefficient of variation is 4.56-10.5% and sensitivity is l μg/24 hr urine. These satisfy the criteria for clinical analysis. It is recommended that the fluorometric method for estrogen determination can be used for evaluation of ovarian activity instead of the tedious spectrophotometric method.
Wang Xuewei, Sun Yonghua, Wei Jianing, Wang Naizuo, Li Yanni, Liu Jiaqi, Li Shengmei
Chinese Medical JournalVol.94,No.031981
DOI: 10.3760/cma.j.issn.0366-6999.1981.03.117
Abstract
Presented in this paper are the surgical considerations in the management of 8 electrically injured patients, with special reference to the care of wrist damage associated with impaired blood flow and to the prospects in handling such accidents. Sound surgical principles such as early diagnosis and repair of artery damage, complete debridement of dead tissues and effective wound closure in conjunction with intensive general treatment and comprehensive treatment planning are necessary for maximally rehabilitated limbs. The patients seem to have benefited a great deal from the procedures outlined, with lowered incidence of amputation and better functional recovery.
This article reports the experience in treating a case of high voltage electric burn of the abdomen complicated by intestinal perforation. The diagnosis and treatment are discussed and a review of the foreign and Chinese literature is given.
Zheng Jianzhong, Chen Zhisheng, Wang Wanmin, Xia Shuzhen
Chinese Medical JournalVol.94,No.031981
DOI: 10.3760/cma.j.issn.0366-6999.1981.03.115
Abstract
The results of acupuncture in 245 cases of retinal arterial occlusion are presented, visual results are divided into 4 grades: marked improvement 24.9%, good improvement 28.6%, slight improvement 33.9% and ineffective 12.6%. Acupuncture is noted for its immediate effect and dramatic improvement in vision in some. Delay in treatment does not seem to affect results in some. The results of acupuncture are superior to medicinal treatment. Three-fourths of the patients preserve the vision achieved at the end of acupuncture treatment during an average follow up period of 3.8 years and two-thirds retain enlargement of the visual field during the same follow up period.
The authors propose the name retroperitoneal extension type of acute necrotic pancreatitis for the condition in which a large amount of exudate containing enzymes flows into the retroperitoneal space. As illustrations the authors report 3 cases of this type in this paper. Early surgical treatment is emphasized and described in detail. In order to differentiate the clinical picture, 38 cases of necrotic pancreatitis and 27 of hemorrhagic pancreatitis are compared.
Indications for closed vitrectomy have increased remarkably during the past 10 years to include numerous anterior and posterior segment conditions not amenable in the past to other forms of intraocular surgery. Instrumentation and technic for both closed and open-sky vitrectomies have undergone numerous improvements, making the procedures relatively safe and results quite encouraging.
Cerebrospinal fluid (CSF) IgG and IgA of 103 cases with disorders of the nervous system without intracranial hemorrhage, CSF IgM of 63 and CSF IgG, IgA and IgM of 9 non-neurologic surgery patients are determined. Increased CSF IgG levels are helpful in the diagnosis of inflammatory demyelinating disorders of the nervous system and increased IgA may also be helpful. CSF concentrated 10 times by polyacrylamide may raise the CSF IgA detection rate. Although the CSF IgG/TP ratio is not a good parameter for the diagnosis of inflammatory demyelinating disorders of the nervous system, dynamic observation of this parameter in individual cases helps the understanding of the tendency of patients' clinical condition to improve or worsen. The mechanism of CSF immunoglobulin increases in various neurologic (especially inflammatory demyelinating) disorders is discussed.
Bladder mucosa flap urethroplasty, a one stage operation for hypospadias repair, is described. Of 110 cases of congenital hypospadias treated by this procedure, 105 (95.5%) were successful. It is indicated for several types of hypospadias and has few complications. For cases with intractable urethral stenosis, the procedure should be preceded by urethrotomy 6 months previously.
Shen Yucun, Zhang Weixi, Shu Liang, Yang Xiaoling, Cui Yuhua, Zhou Dongfeng, Shi Hengyao, Su Entao
Chinese Medical JournalVol.94,No.031981
DOI: 10.3760/cma.j.issn.0366-6999.1981.03.105
Abstract
The prevalence and incidence of severe mental disorders in Beijing agricultural Haidian District population is investigated. The method of investigation and analysis of the data procured is briefly described.
