Volume 107  ·   Issue 02  ·  1994

Anti-idiotypic monoclonal antibody (Mab Ab2) by MAb COC166-9 against ovarian serous papillary adenocarcinoma was prepared. Hybridomas of Ab2 screened by sandwich ELISA and immunocompetitive inhibition tests were procured and named as 6B11 and 1H12. The number of their chromosomes were 93 and 91, and DNA analysis also proved the characteristics of hybridomas. These Ab2s could induce delayed type hypersensitivity (DTH), the cellular immune response. The results of the immune reaction of 6B11 with SKOV3 (ovarian carcinoma cell line) were similar to OC166-9 (Ag), the positive control, while 1H12 was weaker. Anti-anti-idiotypic antibody (Ab3) was also raised by 6B11 and 1H12 respectively. They all showed positive immunohistochemical stainings with ovarian serous adenocarcinoma tissue sections and immunocytochemical stainings with SKOV3 cells as was shown by COC166-9. In the antibody dependent cell mediated cytotoxicity (ADCC) tests, they showed no differences against SKOV3 as compared with COC166-9. We anticipate that 6B11 and 1H12 may be used as vaccines against ovarian carcinoma and may provide a clue for its prevention and treatment.

A young Chinese lady with ACTH-independent Cushing's Syndrome was reported. Her clinical features was compatible with the recently described Carney's complex. Bilateral adrenalectomy was then performed. The diagnosis of bilateral pigmented nodular adrenocortical dysplasia was microscopically confirmed.

The heart weights, ventricular wall thickness and valve circumferences were measured in 507 autopsy specimens of normal hearts from persons aged from 10 to 90 years. The heart weights increased with age and were greater in the males than in the females, except from the 6th to 8th decades where they were reversed. The body weight was the best predictor of heart weight. The ventricular wall thickness in males did not differ significantly from that in females. The values of the indexed left ventricular wall thickness (i.e. values divided by BSA) in the females exceeded those in the males from the 6th to 9th decades. The mean valve circumference was greater in the males but it was significant (P<0.05) only for the tricuspid valves at the 9th decade of life. The pulmonary valve circumferences were greater than the aortic for all decades and the ratio of the aortic to pulmonary valve circumference remained constant. In the clinical evaluation of the specimens of Chinese hearts, the absence of the aged-related dilatation of the aorta described in Caucasian hearts should be duly taken into account.

Among 174 patients with Wolff-Parkinson-White syndrome, 13 (17.5%) had multiple atrioventricular (AV) accessory pathway. Twenty-one of the 29 pathways were identified by baseline electrophysiologic study and eight were found after the radiofrequency ablation of the initial ones. Bilateral ablation approach was tried in four patients and unilateral approach in nine. Twenty-seven of the pathways (93.1%) were eliminated permanently after a mean of 32±14 pulses of current delivery. There were no complications. The pulses was more and the procedure took more time (3.6±0.8 hr) in the group compared to those in single pathway ablation (14±11 and 2.1±0.8 hr respectively, P<0.05). The success rate was identical in the two patient populations (93.1% vs 94.0%, P>0.05). However, the recurrence in multiple pathway ablation (7.6%) was more than that in single pathway ablation (1.9%, P<0.01). Conclusion: radiofrequency catheter ablation is a safe and effective modality for tachyarrhythmias mediated by multiple accessory pathways.

Oligodeoxynucleotides (Oligomers) including modified and unmodified, homo- and heterooligomers were tested for their ability to inhibit mouse SRS leukemia virus (SRSV)-induced proliferation of cells, colony formation, syncytium formation and reverse transcriptase (RT) activity in vitro. Phosphorothioate analogs complementary to Mo-MuLV sequences, as well as noncomplementary homooligomers, were found to be active. Unmodified homooligomer (dC14) also showed inhibition of growth of ascitic lymphoma carrying SRS virus. Our study suggests that different classes of oligonucleotides may inhibit SRSV replication with different mechanisms.

To determine the effect of humoral factors and their interaction on the development of acute hypoxic pulmonary pressor response (HPPR), we performed studies in 16 mongrel dogs. We measured plasma levels of noradrenaline (NE), angiotensin II (AII), prostaglandin F2α (PGF2α), 6-keto-prostaglandin F1α (6KPGF1α), thromboxane B2 (TXB2), leukotriene B4 (LTB4) and 5-hydroxytryptamine (5-HT) before, during and after HPPR. Multiple regression analysis showed that the changes of pulmonary arterial systolic pressure (PASP) and pulmonary arterial diastolic pressure (PADP) correlated well with those of plasma concentration of NE, PGF2α and 6KPGF1α, respectively (r were equal to 0.633 and 0.668, respectively, P<0.01). The results of orthogonal experiment analysis with an injection of exogenous NE, PGF2α and PGIα into main pulmonary artery of dogs showed that NE and the interaction of PGF2α and PGI2α increased PASP (P<0.05) and PGI2 attenuated PASP (P<0.01). The interaction of PGF2α and PGI2 and of PGF2α and NE increased PADP(P<0.01) and PGI2 attenuated PADP (P<0.01).

It is postulated the pulmonary injury in acute necrotizing pancreatitis could be due to oxygen-derived free radicals released during the attack of acute pancreatitis. To elucidate the relationship between oxygen-derived free-radicals and the degree of pulmonary injury in acute necrotizing pancreatitis, lipid peroxide (LPO) in lung tissues, bronchoalveolar lavage fluid and serum were measured in 10 adult Mongrel dogs with acute necrotizing pancreatitis induced by injection of 5% sodium taurocholate into the pancreatic duct. Compared with the controls (n=10), the weight of lung was increased (241.2±33.7 g vs 121.2±51.4 g, P<0.05). LPO of bronchoalveolar lavage fluid (BALF) was higher (3.56±1.64 μmol/L vs 0.75±0.31 μmol/L, P<0.05), LPO in peripheral and portal vein 10 hours after induction of acute pancreatitis was also significantly increased. The LPO content of lung tissue was not higher than that of the control group, but hemorrhage within alveolar space and infiltration by polymorphonuclear and phagocytic cells was seen on histological examination. In conclusion, lipid peroxidation could be an important cause of pulmonary injury in acute necrotising pancreatitis.

Apolipoprotein (a) [Lp(a)] phenotypes of 69 myocardial infarction survivor and 56 stroke patients were reported and compared to those of 190 healthy Chinese. The results revealed that the distributions of apo(a) phenotype frequency in patients with cardio-cerebrovascular disease (CCVD) were different from those of controls. The frequency of the phenotypes S1 and S2 were remarkably higher in patients than in controls within the same single-band apo(a) phenotype. Moreover, the Lp (a) serum concentrations in CCVD patients were significantly higher than in controls within the same single-band apo (a) phenotype. The apo (a) phenotype analysis of two pedigrees were shown as a typical autosomal dominant inheritance.

From September 1985 to December 1992, 160 cases of reconstruction of the esophagus with the whole stomach through the esophageal bed after resection of the upper esophageal carcinoma were performed with neither operative mortality nor intrathoracic complications. The leakage rate of the cervical anastomosis with Gambee's single layer method was 1.2%. The main steps of the operative procedure consisted of: (1) making a right thoracotomy for dissecting and removing the entire thoracic esophagus; (2) laparotomy for mobilizing the whole stomach, constricting it to tube shape and doing a pyloroplasty; and (3) pulling up the mobilized tube-like stomach through the posterior mediastinal space (i.e. the esophageal bed) out of the left neck incision and then the esophagogastrostomy with Gambee's single layer anastomosis was performed.

The Chinese Neurology and Psychiatry Association conducted a national field trial of its Chinese Classification and Diagnostic Criteria of Mental Disorders (CCMD-2) involving 26 provinces and municipalities, 80 psychiatric institutes, and 224 professionals. The results were as follows: (1) 95.2% of researchers considered the comprehensibility of the CCMD-2 diagnostic criteria good. Within the ten major categories, comprehensibility ranged from 85.7% to 100%. (2) Of those surveyed concerning the acceptability of the CCMD-2 diagnostic criteria, 85.9% considered them acceptable. In individual classifications, the rate ranged from 74.1% to 95.2%. (3) 1498 cases were tested. The overall applicability rate which indicated the consistency between the CCMD-2 result and the actual clinical diagnoses was found to be 87.6%, (averaging Kappa=0.82, P<0.01), better than those obtained from non-Chinese systems of diagnosis.

Brain alveococcosis is a rare but threatening disease. In most cases, it metastasized to the brain with late liver or lung alveococcosis. In 14 cases, 8 were secondary, and 6 primary. Nine were treated operatively, 2 medically and 3 proved by autopsy. Eleven cases were monomassive, 2 were multifocal and 1 was extra-cerebral in the cranial base. According to our experiences, extirpation is a radical method, and can improve the cerebral function confirmed by BEAM. Long-period use of albendazole showed calcified lesions of CT and disappearance of clinical symptoms.